Tuesday, February 3, 2009
Antisense in Ripening of Tomato - Animation and ASSIGNMENT 3
My students of SSG1143, please take a good look at the simplified concept of Antisense Technology. Then look up articles in journals that relate to antisense technology as a source of group discussion. Please comment on the article to reflect on your understanding of the subject.
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8 comments:
GROUP 2
From our view about the video is that it gave us all the information we need to know about the antisense. However, there are some explanation that a little confusing. Nevertheless, we can understand better about the antisense process through the video. In our opinion, antisense gives lot of advantages mainly in industrial processes such as in agriculture. Through research we found out that antisense is also applicable in medical, pharmaceutical and others. In general, antisense will bind to the mRNA in order to prevent it from binding to the ribosomes. Thus, translation process cannot occur.
Group 1
The animation on the antisense in ripening of tomato, we have a better idea on how antisense works in a more thorough perspective. Antisense RNA is introduced into a cell to inhibit translation of complementary mRNA. In practise, we can stop the mitosis of cancer cell as for example through the application of antisense RNA. This is due to the synthesis of a strand of antisense RNA which will bind to the mRNA and inactivate it.
GROUP 9
After the long discussion,we find that mRNA antisense can be applied in many sectors for example agriculture, medical aspect,food biotechnology industry and others.
As the same theory for delay ripening of tomato, the shelf life of orchid can also increase by decreasing the production of ethylene using mRNA antisense . Besides that , mRNA antisense can also determine the colour of petals by controlling production of enzymes CHS , DFR and F3-H.We are more understand about antisense after looking trough this few examples.
This animation explains how antisense prevents ripening of tomatoes. From our discussion, we know that there are more industrial antisense applications. For instance, in medical field. Unlike traditional drugs which inactivate viral proteins, antisense RNA blocks the translation of viral mRNA which we think it is more effective in treating patients.
group four,
salam, we are from group four. From animation, we understand about the using of antisense RNA to delay the ripening process of tomato. Antisense RNA is single stranded RNA that is complementary to a mRNA strand transcribed within a cell. Antisense RNA can be introduce into a cell to inhibit translation of a complementary mRNA by base pairing to it and physically obstructing the translation machinery. Furthermore, antisense RNA is useful in other aspects such as agriculture, food technology, medical, pharmaceutical and so on. for example, antisense RNA is being tested experimentally to suppress cancer by stopping chromosome division. Thus, by using antisense RNA, the gene expression or anything else involving RNA can be interfered.
GROUP 7
Antisense technology is a type of biotechnology application to control for gene expression. From the animation posted about the tomato ripening is one of the many functions of antisense technology. We are able to understand how the mechanism of antisense RNA works in order to prevent the tomato ripening. Translation is prevented when there is antisense RNA binds to the mRNA transcribed from the particular selected gene. This antisense RNA is synthesized from gene located downstream at template strand. From the article that our group had chosen, through this kind of controlling mechanism, there are other uses of antisense technology which include enhance the production of recombinant therapeutic glycoproteins in Chinese hamster ovary (CHO) cells, and inhibit transforming growth factor-β, to modulate its effects on scarring during wound healing. Antisense and RNAi agents are useful for cellular bioengineering.
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